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in the episode The Procreation Calculation
Penny: Hey, Leonard, remember yesterday when we were talking about having kids someday? Yeah? Well, what if it wasn't someday.
Leonard: Oh, my God. Oh, my God, are you pregnant?
Penny: No. No. No, no. No, no, no, no, no, no, no, no.
This helps us figure out whether you are a human or a cyborg.