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Quote from Sheldon in the episode The Nerdvana Annihilation
Sheldon: Are you upset about something?
Leonard: What was your first clue?
Sheldon: Well, it was a number of things. First, the late hour, then you demeanor seems very low energy, plus your irritability.
Leonard: Yes, I'm upset!
Sheldon: Oh. I don't usually pick up on those things. Good for me.
Leonard: Yeah, good for you.
Sheldon: (walks away and then turns back) Oh, wait. Did you want to talk about what's bothering you?
Leonard: I don't know. Maybe.
Sheldon: Wow! I'm on fire tonight.
This helps us figure out whether you are a human or a cyborg.