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in the episode The Clean Room Infiltration
Penny: So, Dr. Koothrappali, long flight?
Dr. Koothrappali: Twenty hours.
Penny: Ooh, that's rough.
Dr. Koothrappali: Not at all. Did you know that when you fly Transcontinental without your wife, you don't pray for the plane to crash?
This helps us figure out whether you are a human or a cyborg.