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in the episode The Anxiety Optimization
Sheldon: I'd like to ask you all to do something for me. Keep me on my toes. Just throw me off my game. Essentially, go out of your way to make my life miserable.
Howard: Hold on. What's in it for us?
Sheldon: Well, I suppose-
Howard: Okay, we'll do it!
This helps us figure out whether you are a human or a cyborg.