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in the episode The Separation Oscillation
Sheldon: Why are you up?
Leonard: How am I supposed to sleep? I've been married less than twenty-four hours and my wife isn't speaking to me.
Sheldon: Perhaps you can think of this in a more positive light. In one day, you've managed to do what it takes many couples decades to achieve.
This helps us figure out whether you are a human or a cyborg.