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Quote from Leonard in the episode The Emotion Detection Automation
Raj: You know, I just read a study that suggests new mothers are better at sensing emotions for up to two years.
Amy: It's true. Pregnancy causes physiological changes in the brain that result in increased empathy.
Penny: Huh. So all we need to do is get Sheldon knocked up.
Leonard: We can't. He was already fixed when I found him at the shelter.
This helps us figure out whether you are a human or a cyborg.