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in the episode The Comic-Con Conundrum
Leonard: This is us camping out in line for the Avengers panel.
Penny: Oh, you really sleep on the sidewalk, huh?
Leonard: Yeah. It can get chilly, but Raj figured out that if you pee in a bottle and put it in your sleeping bag, it helps keep you warm.
This helps us figure out whether you are a human or a cyborg.