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in the episode The Desperation Emanation
Sheldon: What makes you chuckle, Leonard?
Leonard: Hmm, recently? Not much.
Sheldon: Is it because of the conflict that arises from your desperate need to pair-bond with a woman, and the apparent collective decision of all womankind to deny you that opportunity?
Leonard: Um, shut up.
This helps us figure out whether you are a human or a cyborg.